Coding

Part:BBa_J119009

Designed by: Cody Barta   Group: Eckdahl Lab   (2011-07-27)


pLacI-RBS-T7RNAP with sites for oligo insertion

J23100 promoter and B0030 RBS upstream of T7 RNAP gene. The following restriction sites do not occur in the promoter-RBS-T7 RNAP sequence. Some of them did not occur in the original sequence and others were removed while conserving the amino acid sequence. Both the forward and reverse complement were checked for type IIs enzyme sites. EcoRI, XbaI, SpeI, PstI, AscI, AsiSI, BsaI, BsmBI, BbsI


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 126
    Illegal BsaI.rc site found at 99


[edit]
Categories
Parameters
None